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Iranian J. of Fisheries Science، جلد ۲۱، شماره ۵، صفحات ۱۲۶۷-۱۲۷۷
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Preparation and biodistribution study of 67Ga-rosemary (Salvia rosmarinus Spenn.) extract nanoparticles and its SPECT imaging in healthy rainbow trout (Oncorhynchus mykiss) |
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| چکیده انگلیسی مقاله |
The aim of this study was to evaluate the labeling of rosemary extract nanoparticles (RE-NPs) using 67Ga radioisotope, and their biodistribution in healthy rainbow trout (Oncorhynchus mykiss) tissues. RE-NPs were prepared by gamma irradiation (irradiated at a dose of 30 kGy of gamma-ray by 60Co (PX-30 IssIedovapel, Russia, dose rate of 0.02 Gy sec-1) and ultra-sonication methods at two concentrations of 0.5% and 1% and labeled by 67Ga radioisotope. Quality control studies were done using the RTLC method. The radiolabeling RE-NPs at 1% concentration showed 97% efficiency using a mixture of sodium acetate and acetic acid buffer at pH=6.5 after 30 min at room temperature. In the biodistribution study, 54 healthy fish were randomly distributed into two treatment groups (n=18 fish/treatment group) and one group served as healthy control. Fish received 3.7-7.4 MBq (300 µL) of radiolabeled RE-NPs at 1% concentration and free 67GaCl3, intraperitoneally. The 67Ga-RE-NPs single-photon emission computed tomography (SPECT) demonstrated the highest tracer accumulation in the kidney and the least uptake in the brain at 24 h after injection of 67Ga-RE-NPs. However, 48 h after injection of 67Ga-RE-NPs, the uptake was negligible in all organs as compared to free 67GaCl3. The obtained results revealed that administration of 67Ga-RE-NPs, as a natural tracer, by IP injection can be a good imaging method to visualize and understand the whole-body, especially the kidney and spleen tissues distribution and pharmacokinetics in healthy rainbow trout. |
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| کلیدواژه‌های انگلیسی مقاله |
rosemary, 67Ga, radiolabeling, Biodistribution studies, Rainbow trout, Gamma Irradiation, SPECT |
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| نویسندگان مقاله |
N. Damavandi Kamali |
Department of Seafood Science and Technology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resource, Gorgan, Iran.
S. Rajabifar |
Nuclear Science and Technology Research Institute, Tehran, Iran.
A.R. Alishahi |
Department of Seafood Science and Technology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resource, Gorgan, Iran.
M. Heidarieh |
Nuclear Science and Technology Research Institute, Tehran, Iran.
S.M. Ojagh |
Department of Seafood Science and Technology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resource, Gorgan, Iran.
S.H. Mirsadeghi |
Department of Seafood Science and Technology, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resource, Gorgan, Iran.
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| نشانی اینترنتی |
https://jifro.areeo.ac.ir/article_128010_9ea3fa1a1e04c88c5c1c7ae960aeeaca.pdf |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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