| چکیده انگلیسی مقاله |
Objective: Neuroprotective and antioxidant effects of Ocimum basilicum (O. basilicum) against pentylenetetrazole (PTZ)-induced seizures were investigated. Materials and Methods: Mice were divided as follows: (Group 1) Control, (Group 2) PTZ, (Groups 3-5) 50,100 and 200 mg/kg hydro-ethanolic (HE) extract, and (Groups 6-8) 200 mg/kg ethyl-acetate (EAF), N-hexane (NHF) and water (WF) fractions. Minimal clonic seizures (MCS) and generalized tonic-clonic seizures (GTCS) latencies were measured. Biochemical and histological studies were done.Results: MCS and GTCS latency in HE groups were longer than the PTZ group (p< 0.05 to p< 0.001). EAF and NHF prolonged the onset of MCS and GTCS (p< 0.001). PTZ increased malondialdehyde (MDA) and dark neuron (DN) production while decreased thiol, catalase (CAT) and superoxide dismutase (SOD) (p< 0.05 to p< 0.001). Pre-treatment by HE and all fractions of the plant attenuated MDA and DN while increased thiol, CAT and SOD (p< 0.01 to p< 0.001).Conclusion: EAF and NHF had anticonvulsant properties. The extract and fractions protected the brain from PTZ-induced oxidative damages and showed neuroprotective effects. |
| نویسندگان مقاله |
| Somaieh Mansouri
Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Anatomy, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
| Mahmoud Hosseini
Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
| Fatemeh Alipour
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
| Farimah Beheshti
Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
Department of Physiology, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
| Hassan Rakhshandeh
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran Science, Mashhad, Iran.
| Abbas Mohammadipour
Department of Anatomy, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
| Akbar Anaeigoudari
Department of Physiology, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran
| Mohammad Jalili-nik
Department of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
| Mohammad Reza Khazdair
Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
| Amirali Jahani
Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran
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