| چکیده انگلیسی مقاله |
Background: Free-living amoebae (FLA) such as Acanthamoeba spp., are considered as opportunistic and pathogenic protozoans. Acanthamoeba granulomatous encephalitis (AGE) is a serious threat for immunodeficient patients and Acanthamoeba keratitis (AK) for contact lens users. We aimed to identify the presence of free living amoebae in nasal swabs of patients and contact lens users in Qazvin, Iran.
Methods: During 2019, 251 nasal and oral swabs (including the pharynx and mouth) were collected from patients with diabetes, AIDS and those under periodic dialysis in Qazvin, Iran. In addition, 27 soft contact lenses were collected from the participants. Following DNA extraction, PCR and sequencing were conducted to identify the genotypes of the amoeba. Phylogenetic analysis of the identified sequences was performed using MEGA 7 software.
Results: A strain of Acanthamoeba belonging to the T3 genotype was isolated from hemodialysis patients. Two specimens of Acanthamoeba with T3 genotype were isolated from keratitis patients.
Conclusion: The clinicians should pay attention to the possible complication of this organism because this amoeba is potentially pathogenic for immunocompromised patients. Since the amoeba is present in environmental resources, the use of contact lenses should be accompanied by considering proper hygiene. |
| نویسندگان مقاله |
| Masoomeh Jalilehvand
Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran
| Elham Hajialilo
1. Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran 2. Department of Parasitology and Mycology, Qazvin University of Medical Sciences, Qazvin, Iran
| Yasaman Ghiasi
Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran
| Saba Rostamian
Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran
| Fatemeh Taherkhani
Department of Biotechnology, Qazvin University of Medical Sciences, Qazvin, Iran
| Fatemeh Kazemi
Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran
| Sima Hashemipour
Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
| Milad Badri
1. Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran 2. Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
| Behzad Bijani
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
| Amir Javadi
Department of Community Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
| Mehrzad Saraei
1. Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran 2. Department of Parasitology and Mycology, Qazvin University of Medical Sciences, Qazvin, Iran
| Sepideh Hajian
Department of Nephrology, Velayat Hospital, Qazvin University of Medical Sciences, Qazvin, Iran
| Peyman Heydarian
1. Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran 2. Department of Parasitology and Mycology, Qazvin University of Medical Sciences, Qazvin, Iran
| Ali Sarbazi Golezari
Metabolic Diseases Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
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