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Iranian Journal of Fisheries Sciences، جلد ۱۹، شماره ۶، صفحات ۳۱۶۰-۳۱۶۹
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Research Article: Molecular diagnosis of pathogenic Aeromonas hydrophila in Cyprinus carpio ulcers |
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| چکیده انگلیسی مقاله |
A total of 150 carp fish were collected from Grdarasha Farm (8 kilometers from Erbil-Iraq) and examined for the presence of pathogenic Aeromonas hydrophila on their skin ulcers, this bacterium secretes a toxin protein called aerolysin which is a virulence factor and has a role in diarrhoeal diseases, deep wound infections and hemorrhagic septicemia. The present study aimed to determine the presence of A. hydrophila in common carp (Cyprinus carpio). For this purpose samples were collected from fish ulcer by swap and incubated at 37°C in blood agar to determine the shape and morphology of colonies, and then the colonies were examined by gram stain test for microscopic examination, finally molecular diagnosis was conducted for aerolysin gene with primer design and PCR. According to the diagnosis methods results were different; PCR diagnosis confirmed that only 68 skin lesions were caused by A. hydrophila, no visceral lesion was caused by A. hydrophila and this bacterium hardly ever seen in the skin surrounding mouth as only 2 lesions surrounding mouth had A. hydrophila in PCR diagnosis. |
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| کلیدواژه‌های انگلیسی مقاله |
Aerolysin, Aeromonas hydrophila, Cyprinus carpio, Polymerase chain reaction |
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| نویسندگان مقاله |
| I.R. Ibrahim
Fish Recourses and Aquatic Animals Department, College of Agriculture, Salahaddin University-Erbil, Erbil, Iraq.
| S.M.A. Abdullah
Fish Recourses and Aquatic Animals Department, College of Agriculture, Salahaddin University-Erbil, Erbil, Iraq.
| A.Y. Karim
Biology Department, College of Science, Salahaddin University-Erbil, Erbil, Iraq.
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| نشانی اینترنتی |
http://jifro.ir/browse.php?a_code=A-10-3000-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
fish disease |
| نوع مقاله منتشر شده |
پژوهشی |
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