|
|
Iranian Journal of Fisheries Sciences، جلد ۲۰، شماره ۶، صفحات ۱۸۰۴-۱۸۲۱
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Research Article: Comparison of glutaraldehyde cross linking versus direct Schiff base reaction for conjugation of L-asparaginase to nano-chitosan and improvement of enzyme physicochemical properties |
|
| چکیده انگلیسی مقاله |
The bacterial L-asparaginase (ASNase) has been used in the treatment of asparagine-associated tumors; however, the instability of the enzyme increases the number of injections as well as the side effects. In the present study, ASNase was conjugated to nanochitosan (ASNase -CSNPS) by direct (shiff-base) and indirect (glutaraldehyde linker) methods. In order to get the optimal conjugation, ASNase/CSNPS ratio was first investigated. The physicochemical properties (optimum pH, temperature, residual activity), enzyme kinetics (Michaelis constants; Km and maximal velocity; Vmax) and stability (against freezing, proteolysis, and chemical denaturation) were determined. The results showed that the highest residual enzyme activity (>85%) was obtained using a combination of ASNase and CSNPS at 1:5 mass ratio in both conjugation methods. ASNase -CSNPS prepared by glutaraldehyde linker had higher Km and Vmax values (69.7 μM, 20.6 mol/mL/minμ), wider range of optimum pH and higher temperature stability compared to ASNase-CSNPS produced by Schiff-base (Km: 105.8 μM, Vmax 14.5 mol/ml/minμ) method. ASNase-CSNPS produced by indirect method had more stability against freezing-thawing, and proteolysis when compared with ASNase-CSNPS prepared using direct method. The results showed that application of glutaraldehyde coupling was superior to Schiff base cross linking for conjugating of ASNase to CSNPS and for production of ASNase with better physicochemical properties for future cancer therapy. |
|
| کلیدواژه‌های انگلیسی مقاله |
L-Asparaginase, Nanochitosan, Glutaraldehyde linker, Schiff base reaction, physicochemical properties |
|
| نویسندگان مقاله |
| N. Khounmirzaie
Department of Veternary, Babol Branch, Islamic Azad University, Babol, Iran
| M. Razijalali
Department of Veternary, Babol Branch, Islamic Azad University, Babol, Iran
| A. Shahriari
Department of Veternary, Babol Branch, Islamic Azad University, Babol, Iran
| M.R. Tabandeh
Department of Veternary, Babol Branch, Islamic Azad University, Babol, Iran
|
|
| نشانی اینترنتی |
http://jifro.ir/browse.php?a_code=A-10-4199-1&slc_lang=en&sid=1 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Biochemistry |
| نوع مقاله منتشر شده |
پژوهشی |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|