Iranian Journal of Fisheries Sciences، جلد ۲۰، شماره ۶، صفحات ۱۵۴۲-۱۵۵۹
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عنوان انگلیسی Research Article: Biotyping and serotyping of Lactococcus garvieae isolates in affected farmed rainbow trout (Oncorhynchus mykiss) in north Iran
چکیده انگلیسی مقاله The incidence of Lactococcosis caused by Lactococcus garvieae, in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) farms is disquieting and increasing in recent years. Knowledge of biotypes and serotypes involved in disease incidence is essential to adopt disease prevention and control policies. Twelve isolates of L. garvieae from some rainbow trout farms were identified by phenotypic, biochemical, and molecular assays. Biotyping of the isolates was undertaken based on the acidification of carbohydrates including sucrose, tagatose, mannitol, and cyclodextrin as well as the presence of pyroglutamic acid arylamidase (Pyra) and N-acetyl-β-glucosaminidase (β-Nag) enzymes. The Serotyping classification was carried out using slide agglutination assay. The obtained results showed that all isolates were identified as biotype II. Seven isolates (58.3%) were positive for capsule formation and were classified as KG- strain and 41.7 % were classified as KG+ strains (non-capsulated strains). Further works are required for a better understanding of the diversity of Lactococcus garvieae isolates in farmed rainbow trout in Iran.
کلیدواژه‌های انگلیسی مقاله Lactococcus garvieae, Rainbow trout, Biotyping, Serotyping, Capsule
نویسندگان مقاله | M. Soltani
Univsersity of Tehran


| N. Naeiji
University of Tehran


| A. Zargar
University of Tehran


| P. Shohreh
Univerwsity of Babol


| A. Taherimirghaed
University of Tehran
نشانی اینترنتی http://jifro.ir/browse.php?a_code=A-10-776-10&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده fish disease
نوع مقاله منتشر شده پژوهشی
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