|
|
مجله دانشگاه علوم پزشکی فسا، جلد ۱۱، شماره ۴، صفحات ۴۰۸۸-۴۰۹۹
|
|
|
| عنوان فارسی |
اثرات محافظتی ویتامین D بر روی بافت بیضه ، استرس اکسیداتیو و بیان ژنهای Bcl۲ ، Bax ، caspase-۳ و p۵۳ در موشهای صحرایی تحت درمان با سیمواستاتین |
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Protective Effects of Vitamin D on Testicular Tissue, Oxidative Stress and Apoptotic Genes Expression in Rats Treated with Simvastatin |
|
| چکیده انگلیسی مقاله |
Background & Objective: Cardiovascular diseases (CVDs) are the deadliest disease globally, which in order to reduce this mortality rate statin has been used during last decades. In line, it has been revealed that statins negatively affect testicular tissue. Thus, in the current project, we aimed to investigate the vitamin D protective effects during simvastatin (SIM) administration. Materials & Methods: In this project, 48 male mature Wistar rats were randomly divided into 6 control and experimental groups. The experimental groups were subdivided into Low and High dose SIM-received and Vitamin D co-treated SIM-received groups. After 45 days, the body weight of the animal groups was measured and then they were euthanized and testicles were removed. The right testicular tissue was fixed in fixative solution, while the left testicular tissue was used for biochemical and molecular analysis. To evaluate the pathological changes, hematoxylin and Eosin (H&E) staining were performed. The Malondialdehyde (MDA), Total antioxidant capacity (TAC), catalase (CAT) and Ferric-reducing antioxidant power (FRAP) levels were examined in order to evaluate the Reactive oxygen species (ROS) and antioxidant levels. Finally, Bax, Bcl-2, p53 and Caspase-3 mRNA levels were analyzed using real- time PCR. Result: Our observations revealed that the MDA, Bax, p53 and caspase-3 level enhanced in the sole-SIM-received groups compared to control group, while level of TAC, catalase and Bcl-2 diminished in SIM-received groups versus control group. In contrast, Vitamin D-co-treated animals showed a significant enhancement in level of TAC, catalase and Bcl-2 compared to sole-SIM-received animals (p≤0.05). Conclusion: The vitamin D-co-administration can positively protect testicular tissue against SIM-induced oxidative stress condition by enhancing enzymatic activity of antioxidant defense and anti-apoptotic genes activation. |
|
| کلیدواژه‌های انگلیسی مقاله |
Antioxidant, Apoptosis, Simvastatin, Stress oxidative, Vitamin D |
|
| نویسندگان مقاله |
ادریس کیاراد | Kiyarad Edris
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
گروه زیست شناسی، دانشکده علوم، دانشگاه ارومیه، ارومیه، ایران
غلامرضا نجفی تازه کند | Gholamreza Najafi Tazekand
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
گروه آناتومی و جنین شناسی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران
فرح فرخی | Farah Farrocki
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
گروه زیست شناسی، دانشکده علوم، دانشگاه ارومیه، ارومیه، ایران
|
|
| نشانی اینترنتی |
http://jabs.fums.ac.ir/browse.php?a_code=A-10-2804-1&slc_lang=en&sid=1 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
بافت شناسی |
| نوع مقاله منتشر شده |
پژوهشی |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|