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Middle East Journal of Cancer، جلد ۱۲، شماره ۲، صفحات ۲۰۸-۲۱۸
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عنوان انگلیسی Armeniacae Semen Regulates Apoptosis and Cell Cycle Progression in MCF-7, MDA-MB-231, and T47D Breast Cancer Cell Lines
چکیده انگلیسی مقاله Background: Amygdalin is a glycoside ingredient of rosacea plants that exerts an antitumor effect by blocking the growth of the tumor cells. Therefore, we aimed to use the Armeniacae semen, a member of the Rosacea family, which contains a large amount of the amygdalin, to evaluate its antiproliferative effect on MCF-7, MDA-MB-231, and T47D breast cancer cell lines. Method: In this experimental study, we prepared the aqueous, ethyl acetate, and hydro-alcoholic extracts of the Armeniacae semen. The MCF-7, MDA-MB-231, and T47D cell lines were treated with different doses of the extracts for 12, 24, 36, and 48 hours; cell viability was investigated with MTT test and cell apoptosis was detected by use of double staining fluorescent. Cell cycle progression was analyzed using a BD Cycle TEST PLUS DNA Kit. We also assessed Bcl2, Bax, and caspase-3 mRNA expression. Results: The best IC50s belonged to hydro-alcoholic extract of the Armeniacae semen in all three cell lines for the 48-hour treatment. We observed a significant increase in Bax and caspase-3 mRNA expression and a noticeable reduction in Bcl2 mRNA compared with the controls. Application of amygdalin to MCF-7, MDA-MB-231, and T47D cell lines increased the number of G0/G1 cells and reduced the number of cells at G2/M phasecompared to the controls. Conclusion: This study showed that the hydro-alcoholic extract of Prunus armeniaca had antitumor effects on breast cancer cell lines as it inhibited the cell cycle at G0/G1 phase and apoptosis induction in the MCF-7, MDA-MB-231, and T47D cell lines.
کلیدواژه‌های انگلیسی مقاله Armeniacae semen, Breast neoplasms, Cell Cycle, Caspase 3, Proto-Oncogene Proteins c-bcl-2, Bax
نویسندگان مقاله Shiva Mosadegh Manshadi |
Department of Hematology and Blood Banking, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran

Fatemeh Nadali |
Department of Hematology and Blood Banking, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran

Mohammad Reza Shams Ardekani |
Department of Pharmacognosy and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

نشانی اینترنتی https://mejc.sums.ac.ir/article_47038_1ed804964b0adc624fe6a5e42b40d950.pdf
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