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Iranian Journal of Public Health، جلد ۵۰، شماره ۱۰، صفحات ۲۱۱۱-۲۱۲۰
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عنوان انگلیسی Photodynamic Therapy Using Toluidine Blue O (TBO) Dye as a Photosensitizer against Leishmania major
چکیده انگلیسی مقاله Background: Photodynamic therapy (PDT) is alternative treatment of cutaneous leishmaniasis (CL), and phenolthiazine dyes such as Toluidine Blue O (TBO) have the potential role in PDT and notably affect parasites inactivation. This study aimed to evaluate the effectiveness of PDT by using TBO and a light-emitting diode (LED) in the treatment of zoonotic CL (ZCL). Methods: The study was conducted in Iran University of Medical Sciences, Tehran, Iran in 2018-2020. Different concentration (7.8 µg/ mL up to 500 µg/ mL) of TBO as a photosensitizer and a 630 nm LED light as a source of light were used for antileishmanial activity against both forms of Leishmania major promastigotes and intracellular amastigotes. Effective concentration (EC50) and cell cytotoxicity (CC50) were calculated in both infected and non-infected J774.A1 macrophages, respectively. As well as inhibitory concentration (IC50) was quantified in L. major promastigotes for 2 h, 24 h, and 48 h after incubation using a MTT colorimetric assay. Results: TBO dye in combination with the PDT significantly decreases the L. major promastigotes and intracellular amastigotes viability when compared with TBO alone. Both TBO dye in combination with the PDT and TBO alone had no toxic effects on the mice macrophages; however, it significantly killed the entered parasites inside the cells. Our results in the current study established satisfactory findings in clearing intracellular L. major parasites in in-vitro conditions. Conclusion: TBO dye in combination with the PDT can be considered as a harmless, effective and importantly perfect treatment against L. major, causative agent of ZCL, in an in-vitro situation without any negative toxicity to the mice macrophages.
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نویسندگان مقاله | Mehdi Najm
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Maryam Pourhajibagher
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran


| Alireza Badirzadeh
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Elham Razmjou
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Maryam Alipour
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Majid Khoshmirsafa
Immunology Research Center (IRC), Iran University of Medical Sciences, Tehran, Iran


| Abbas Bahador
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Ramtin Hadighi
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی https://ijph.tums.ac.ir/index.php/ijph/article/view/20768
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