|
|
Physiology and Pharmacology، جلد ۲۵، شماره ۱، صفحات ۷۶-۸۲
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Time course study of ERK1/2 activity and cell viability in lipopolysaccharide challenged PC12 cells |
|
| چکیده انگلیسی مقاله |
Introduction: Extracellular signal-regulated kinase 1 and 2 (ERK1/2) are important members of mitogen-activated protein kinases (MAPK), which are actively involved in the shaping of cellular responses to different stimuli; however, these responses are somehow contradicting. It is believed that time is a crucial factor in the determination of these effects. Therefore, the present work was designed to obtain a more vivid view about the effect of time on ERK activity and its relation to cell viability. Methods: In the first step, we challenged cultured PC12 cells with different doses of lipopolysaccharides (LPS) for different time intervals (3, 6, 24 and 48h) and the cell viability was checked by MTT test. Thereafter, we cultured the cells in 6-well plates and treated them with the effective dose (10μg/ml) for the abovementioned intervals and the level of ERK phosphorylation, as the active form, was assessed in the Western blotting analysis. Results: The results showed that treating cells with 10μg/ml LPS reduces cell viability after 48h. While being ineffective in shorter periods of time, ERK activity has a fluctuating trend, so that it reaches the highest level at 6h, thereafter it declines to the lowest level at 24h and partially increases again at 48h. Conclusion: These results imply that time is a determinant factor in the activity of ERK and single-point assessments may result in misinterpretation. |
|
| کلیدواژه‌های انگلیسی مقاله |
Inflammation, Time factors, ERK1/ 2 MAPK, Lipopolysaccharides, PC12 cells. |
|
| نویسندگان مقاله |
| Pegah Javadpour
Department of Physiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| Sahar Askari
Department of Physiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| Fateme Azizi
School of advanced medical technologies, Tehran university of medical sciences, Tehran, Iran
| Rasoul Ghasemi
Department of Physiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
|
|
| نشانی اینترنتی |
http://ppj.phypha.ir/browse.php?a_code=A-10-1079-1&slc_lang=en&sid=1 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Cell, Stem Cell and Cancer |
| نوع مقاله منتشر شده |
Experimental research article |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|