| چکیده انگلیسی مقاله |
Microsatellite markers were used to identify informative markers associated with traits lattice shape of pod, belly shape in pod, pod length, pod width, grain number per pod, grain weight, grain weight/pod weight, grain length, grain width, pod number per plant, grain number per plant, pod weight per plant, grain weight per plant in peanut. Thirteen SSR primer pairs amplified 80 alleles among 68 peanut genotypes, with an average of 6.15 alleles per locus. Polymorphic information content ranged from 0.47 (Locus pPGPseq-2C11) to 0.95 (Locus PM183), with an average of 0.80. Stepwise regression analysis between molecular data as independent variables, and morphological data as dependent variables was performed to identify informative markers associated with the studied traits. SSR loci associated with number of grains per plant and weight of pod per plant were the same. Loci Ah4-4, Lec and PM210 were associated with both grain length and width. The most variation of grain length (88%) was accounted by Ah4-4, Ah4-26, Lec, PM3, PM36, PM50, PM183, PM210, pPGPseq-2D12B and Ah51 markers while Ah4-4, Lec and PM210 markers were accounted for 26% of the variation of the grain width. Since all the used SSR loci except pPGPseq-2C11 showed significant association with the studied traits, therefore, it is possible to use these markers along with morphological traits in peanut breeding programs for identification of suitable parents to produce mapping populations and hybrid varieties. |