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فصلنامه علمی پژوهشی گیاهان دارویی، جلد ۳، شماره ۷۱، صفحات ۷۷-۸۴
عنوان فارسی
چکیده فارسی مقاله Background: Dorema aucheri (Apiaceae) grows in west and southwest of Iran. Young leaves of this plant are used orally as pickle or for cooking some local foods, as well as for the treatment of parasitic digestive infections and constipation. Objective: The aim of present study was evaluation of cytotoxic potential and total phenols determination of D. aucheri young aerial parts extract and fractions and its pickle extract. Method: The dried powders of D. aucheri young aerial parts and pickles were macerated with 80% methanol and fractionated with petroleum ether (PE), chloroform (CL), ethyl acetate (EA) and methanol (ME). Cytotoxic activity of the total extract, fractions and pickle extract on HT-29, MDA-MB-231, A549, Hela cancer and normal fibroblast cell lines were assessed by MTT assay. Total phenol contents of all samples were determined by Folin-Ciocalteu method. Results: The potent cytotoxic activities were shown by PE and CL fractions on normal fibroblast cell line with IC50 equal to 17.66±2.3 and 48.80±1.24 µg/ml, respectively. Other fractions and extracts didn't have considerable cytotoxic effects on other cell lines. In addition, two mentioen fractions had the lowest total phenols. ME and EA fractions with highest amounts of phenols showed the lowest cytotoxicity. Conclusion: It is concluded D. aucheri compounds have potential to exhibit toxic effects on healthy cells. On the other hand, there is negative correlation between cytotoxicity and phenol contents of D. aucheri fractions. 77
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Alert for Consumption of Dorema aucheri: an Edible Medicinal Plant of Iran
چکیده انگلیسی مقاله Background: Dorema aucheri (Apiaceae) grows in west and southwest of Iran. Young leaves of this plant are used orally as pickle or for cooking some local foods, as well as for the treatment of parasitic digestive infections and constipation. Objective: The aim of present study was evaluation of cytotoxic potential and total phenols determination of D. aucheri young aerial parts extract and fractions and its pickle extract. Method: The dried powders of D. aucheri young aerial parts and pickles were macerated with 80% methanol and fractionated with petroleum ether (PE), chloroform (CL), ethyl acetate (EA) and methanol (ME). Cytotoxic activity of the total extract, fractions and pickle extract on HT-29, MDA-MB-231, A549, Hela cancer and normal fibroblast cell lines were assessed by MTT assay. Total phenol contents of all samples were determined by Folin-Ciocalteu method. Results: The potent cytotoxic activities were shown by PE and CL fractions on normal fibroblast cell line with IC50 equal to 17.66±2.3 and 48.80±1.24 µg/ml, respectively. Other fractions and extracts didn't have considerable cytotoxic effects on other cell lines. In addition, two mentioen fractions had the lowest total phenols. ME and EA fractions with highest amounts of phenols showed the lowest cytotoxicity. Conclusion: It is concluded D. aucheri compounds have potential to exhibit toxic effects on healthy cells. On the other hand, there is negative correlation between cytotoxicity and phenol contents of D. aucheri fractions.
کلیدواژه‌های انگلیسی مقاله Bilhar, Cytotoxic, MTT assay, Phenolics, Umbelliferae
نویسندگان مقاله | M Eftekhari
School of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran


| F Oskou
School of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran


| Z Tofighi
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, 14174-14411, Tehran, Iran


| E Motevaseli
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran


| S Jafari Nodooshan S
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran


| MR Shams Ardekani
School of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://jmp.ir/browse.php?a_code=A-10-2063-2&slc_lang=en&sid=1
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/88/article-88-1927472.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده داروسازی
نوع مقاله منتشر شده پژوهشی
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