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Veterinary Research Forum، جلد ۳، شماره ۴، صفحات ۲۵۷-۲۶۱
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عنوان انگلیسی Phenotypic modulation of auto-reactive cells by insertion of tolerogenic molecules via MSC-derived exosomes
چکیده انگلیسی مقاله Auto-reactive cells-mediated immune responses are responsible for the current tissue damages during autoimmunity. Accordingly, functional modulation of auto-reactive cells has been a pivotal aim in many of recent studies. In the current study, we investigated the possibility for insertion of regulatory molecules onto auto-reactive cells through exosomal nano-shuttles as a novel approach for phenotype modification of auto-reactive cells. The exosomes were isolated from supernatant of mesenchymal stem cells culture. Resultant exosomes co-cultured with lymphocytes were harvested from established EAE mice in the presence of antigenic MOG35-55 peptide. After 24 hr, insertion of exosomal tolerogenic molecules (PD-L1, TGF-β, galectin-1) onto auto-reactive cells were explored through flow cytometry. The potency of exosomal inserted membrane molecules to modulate phenotype of auto-reactive lymphocytes was assessed upon ELISA test for their-derived cytokines IFN-γ and IL-17. Incorporation of exosomal molecules into lymohocytes’ membrane was confirmed by flow cytometric analyses for surface levels of mentioned molecules. Additionally, the decreased secretion of IFN-γ and IL-17 were detected in exosome pre-treated lymphocytes upon stimulation with MOG peptide. Mesenchymal stem cells -derived exosomes showed to be efficient organelles for insertion of bioactive tolerogenic molecules onto auto-reactive cells and modulation of their phenotypes.
کلیدواژه‌های انگلیسی مقاله Auto-reactive cell,EAE,MSC,Exosome,Tolerogenic molecule
نویسندگان مقاله آرام مکاری زاده |
department of microbiology, faculty of veterinary medicine, urmia university, urmia, iran
سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

نوروز دلیرژ |
department of microbiology, faculty of veterinary medicine, urmia university, urmia, iran
سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

ahhmad مرشدی |
department of microbiology, faculty of veterinary medicine, urmia university, urmia, iran
سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

قاسم مسیبی |
department of immunology and microbiology, faculty of medicine, arak university of medical sciences, arak, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اراک (Arak university of medical sciences)

بهرام دلیرنقده | dalir naghadeh
department of clinical sciences, faculty of veterinary medicine, urmia university, urmia, iran
سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

نشانی اینترنتی http://vrf.iranjournals.ir/article_1599_10fb148afd84aad319ac66f668fdf370.pdf
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