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Iranian Journal of Parasitology، جلد ۱۴، شماره ۱، صفحات ۲۰-۲۸
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عنوان انگلیسی Serological and Molecular Diagnosis of Toxoplasma gondii Infections in Thalassemia Patients
چکیده انگلیسی مقاله Background: This study aimed to the serological and molecular diagnosis of Toxoplasma gondii infections and related risk factors in patients with thalassemia major and healthy controls. Methods: This case-control study was performed in Shahrekord University of Medical Sciences, Shahrekord, west of Iran from Jan 2014 to Jan 2015. Overall, 235 patients with thalassemia major and 235 healthy controls were enrolled. Assessment of anti-Toxoplasma antibodies in sera samples was performed using commercial ELISA kits. In order to the molecular investigate of T. gondii in blood samples, a relatively new molecular assay, LAMP technique based on Toxoplasma SAG1 gene was conducted for the first time. The specificity of LAMP outer primers for the T. gondii detection was confirmed by sequencing the purified PCR product. Results: 51.9% of thalassemia patients and 34.8% of healthy controls were positive for anti-Toxoplasma IgG antibodies, which the difference was statistically significant (P< 0.01). In terms of anti-Toxoplasma IgM antibody, 3.4% of thalassemia patients and 2.1% of healthy individuals were positive, which the difference was not statistically significant (P=1). Based on SAG1-LAMP, 9.78% of the thalassemia patients and 5.95% of healthy controls were positive for T. gondii DNA, which the difference was not statistically significant (P≤0.230). Conclusion: Thalassemia patients, probably due to repeated blood transfusion and consequently, immune deficiency, are at risk of transmitting Toxoplasma infection more than healthy people. Therefore, screening of Toxoplasma infection in blood transfusion centers may be effective in the prevention of toxoplasmosis in these patients.
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نویسندگان مقاله | Hooman HANIFEHPOUR
Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran


| Seyed Kamal SAMSAM SHARIAT
Department of Medical Parasitology and Mycology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran


| Mohammad Saleh GHAFARI
Department of Medical Parasitology and Mycology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran


| Farnaz KHEIRANDISH
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran


| Vafa SABER
Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Shirzad FALLAHI
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1337
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-1353721.pdf
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