|
|
Iranian Journal of Fisheries Sciences، جلد ۱۳، شماره ۴، صفحات ۸۹۵-۹۰۶
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Identification of a sex-linked SCAR marker for Plecoglossus altivelis and its application for identifying gender in cultivated and wild populations |
|
| چکیده انگلیسی مقاله |
Ayu (Plecoglossus altivelis), one kinds of valuable cultured fish species, show almost no morphological difference between male and female until sexual maturity. Here, we report the identification of sex-linked markers for the ayu, based on Amplified Fragment Length Polymorphism (AFLP) generated from cultured fish (15 males and 15 females) by using 63 different primer combinations. Genomic fragments (n = 3733) were produced with a mean frequency of 59 bands per primer pair. A male-specific of 139 base pair band was amplified and converted to a sequence-characterized amplified region (SCAR marker) designated as Ayu102. Six distinct genomic fragments were produced in 12 wild samples (6 males and 6 females). The fragments designated a, b, c, d, and e were detected only in males, and one fragment (f) was detected in both genders. Nucleotide sequence analysis showed that fragments e and f were 96.67% identical. Ayu102 marker was detected in 45-d-old larvae, in both cultured and wild fish populations, and in offsprings generated by gynogenesis. Fragment e was detected in all males, and in only 6.7% of females in cultured fish. Data suggest that Ayu102 marker is a male-specific marker linked to the sex-determining locus of the male ayu and can be used for gender identification. |
|
| کلیدواژه‌های انگلیسی مقاله |
AFLP, Plecoglossus altivelis, Sex-linked marker. |
|
| نویسندگان مقاله |
c ونگ | c wang
| j wang
| j wang
| s iranshahi
| s iranshahi
|
|
| نشانی اینترنتی |
http://www.jifro.ir/browse.php?a_code=A-10-1272-116&slc_lang=en&sid=en |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Biology & physiology |
| نوع مقاله منتشر شده |
پژوهشی |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|