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Iranian Journal of Fisheries Sciences، جلد ۱۵، شماره ۴، صفحات ۱۲۷۹-۱۲۹۲
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Isolation and phylogenic analysis of emerging new antibiotic resistant bacteria, Acinetobacter lwoffii, associated with mortality in farmed rainbow trout |
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| چکیده انگلیسی مقاله |
Whereas it is well documented that the genus Acinetobacter is associated with antibiotic resistant bacteria and human clinical infections, there are very few studies to date that report it as an emerging new pathogen for fish. In the present study, Acinetobacter lwoffii was isolated from diseased rainbow trout (Oncorhynchus mykiss) from Iranian farms, in the Khuzestan Province by both biochemical tests and polymerase chain reaction (PCR)-based methods and further confirmed as the causative agent by infectivity experiment. Uni or bilateral exophthalmia, blackening of the skin, abdominal distension, hemorrhages around the mouth cavity and eyes, basal fine, skin, gills and in internal organs were the main clinical signs in the affected fish. Phylogenetic analysis of the 16S rRNA sequence of MD77 revealed a 99% homology with that of A. lwoffii (GenBank accession no.KR856323.1) form Iran. Histopathological changes of challenged rainbow trout including focal necrosis of liver cells (hepatocyte), pyknotic nucleus and karyolysis of hepatocyte, hyperemia, fatty acid composition change of liver, infiltration of inflammatory cells, sinusoidal dilatation and congestion. The drug resistance of isolates and minimal inhibitory concentration (MIC) was examined and showed that A. lwoffii was multiresistant to 8 of the antimicrobial agents tested.. |
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| نویسندگان مقاله |
m دادار | m dadar
razi vaccine and serum research institute, agricultural research, education and extension organization areeo , karaj, i.r. iran
سازمان اصلی تایید شده: موسسه تحقیقات واکسن و سرم سازی رازی (Razi vaccine and serum research institute)
m عادل | m adel
department of aquatic animal health amp;amp; disease, iranian fisheries science research institute ifsri , agricultural research education and extension organization areeo , p.o.box 13185-116, tehran, iran
محمدجواد ذریه زهرا | m j zorriehzahra
department of aquatic animal health amp;amp; disease, iranian fisheries science research institute ifsri , agricultural research education and extension organization areeo , p.o.box 13185-116, tehran, iran
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| نشانی اینترنتی |
http://www.jifro.ir/browse.php?a_code=A-10-335-8&slc_lang=en&sid=en |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
fish disease |
| نوع مقاله منتشر شده |
پژوهشی |
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