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Iranian Journal of Chemistry and Chemical Engineering، جلد ۲۵، شماره ۴، صفحات ۷۳-۷۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Determination of Juglone (5-hydroxy 1,4-naphthoquinone) in Pterocarya fraxinifolia by RP-HPLC |
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| چکیده انگلیسی مقاله |
A method for determination of juglone in leaves and hulls of Pterocarya fraxinifolia was developed, based on RP-HPLC. This compound was extracted from leaves and hulls by chloroform refluxing about 2 hours. The chloroform was evaporated under low pressure at 50 ºC to produce a dry residue. The remained materials were dissolved in acetonitrile to be used for determination of this compound. Appropriate conditions for RP-HPLC were determined using standard of juglone. These conditions include, acetonitrile percentage in mobile phase, pH of mobile phase (phosphate buffer) and temperature of column, which were 50%, 4 and 30ºC, respectively. Juglone concentration was determined using standard addition method. The concentrations of juglone were 2.15, 2.74,1.77,1.12and 0.34 g in 100 g of dry leaves in May, June, July, August and September 2001, respectively. The content of juglone in hulls was 0.44 g in 100 g of dry hulls in May. |
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| نویسندگان مقاله |
محمدرضا hadjmohammadi | mohammad reza
department of chemistry, university of mazandaran, p.o.box 453 babolsar, i.r. iran
سازمان اصلی تایید شده: دانشگاه مازندران (Mazandaran university)
کامیار کامل |
department of chemistry, university of mazandaran, p.o.box 453 babolsar, i.r. iran
سازمان اصلی تایید شده: دانشگاه مازندران (Mazandaran university)
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| نشانی اینترنتی |
http://www.ijcce.ac.ir/article_8034_282379365eeaf7d658e15ff3dc4cd0d1.pdf |
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| زبان مقاله منتشر شده |
en |
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