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International Journal of Pediatrics، جلد ۲، شماره ۲.۳، صفحات ۸۷-۸۷
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عنوان انگلیسی Toxicity Study of Gelatin-chitosan Films with Bone Marrow Stromalcells Cultured in Rat
چکیده انگلیسی مقاله Background Gelatin and chitosan are known as biodegradable and biocompatible biopolymers.These biopolymers have recently receivedincreasingly more attention for tissueengineering.The aim of this study was to evaluate the toxicity effects of gelatin-chitosan film on bone marrow stromal cell(BMSCs) culture in rat. Material & Methods: First, gelatin- chitosan composites film were prepared by solution mixing, followed by film casting of both biopolymers in glacial acetic acid.After two passage of BMSCs culture, the cells were cultured in four plate groups including: control plates have no any film,gelatinplates,chitosan plates and gelatin- chitosan plates.Theproliferation,differentiation,viability and apoptosis rates of BMSCs were studied duringthesecond,fourthand sixth days.The activity of superoxidedismutase(SOD),catalase(CAT)and glutathione(GSH) and malondialdehyde(MDA) levels were determined by using biochemical methods. Results: The results showed that the mean BMSCs proliferation significantly reduced in chitosan group compared to control group (p< 0.05),but in gelatin and gelatin- chitosan groups were similar to control group.Mean percentage of BMSCs apoptosis in all groups except chitosan group were similar to control group. Mean percentage of BMSCs viability at all groups was similar to control group except chitosan film. In addition, no significant changes were observed in CAT activity and GSH and MDA levels in comparison with control group.After72hours,SOD activity in gelatin-chitosan group was significantly reduced compared to other groups. No cell differentiation was detected in all groups. Conclusion: Results of proliferation, differentiation, apoptosis and antioxidantactivity in cultured BMSCs on a gelatin–chitosanfilm showed that gelatin-chitosanfilm can be used in tissue engineering and cell therapy as a good model of a biodegradable scaffold. Keywords: Antioxidant system, Bone Marrow Stromal Cells, Cell Proliferation andDifferentiation, Gelatin-Chitosan Film.
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نویسندگان مقاله افسانه کرمی جویانی | afsane karami juyani
researcher, aja university of medical siences, tehran, iran.


مهدی صابری | mehdi saberi
proffesor, department of pharmacology, baqiyatallah university of medical sciences

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

غلامرضا کاکا | gholamreza kaka
assistant professor, neuroscience research center, baqiyatallah university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

مهوش جعفری | mahvash jafari
assistant professor, department of biochemistry, baqiyatallah university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

سیمین ریاحی | simin riyahi
phd of exercise physiology, instructor, aja university of medical siences, tehran, iran.


ملیحه تقی زاده | maliheh taghizadeh
phd candidate of medical university, tabiat modares university. instructor, aja university of medical siences, tehran, iran.
نشانی اینترنتی http://ijp.mums.ac.ir/article_2761.html
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