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International Journal of Pediatrics، جلد ۲، شماره ۲.۳، صفحات ۵۲-۵۲
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| عنوان انگلیسی |
Ectopic Expression of Embryo/Cancer Sequence A (ECSA) in KYSE-30 Cell Line Using Retroviral System |
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| چکیده انگلیسی مقاله |
Background Human preimplantation embryonic cells share many similarities with cancer cells such as ability to self-renew, unlimited proliferation and maintenance of the undifferentiated state. Embryo-cancer sequence A (ECSA), also known as developmental pluripotency associated-2 (DPPA2), is a cancer testis antigen (CTA) with unclear biological function yet. Objective: CTAs are expressed normally in germ line cells and trophoblast, and aberrantly in a variety of cancers. According to the importance of ECSA in developmental events and cancer, preparing a suitable platform to analyze its roles seems necessary. Methods The coding sequence of the gene was amplified and sub-cloned in pRUF retroviral expression vector. pRUF- ECSA vector was cotransfected with pVSV-G to GP293 cells and with pVSV-G and pGP to HEK293 packaging cell lines. Then the viral particles were transducted to KYSE-30 cells and the concentration of retroviral particles was determined by Real time PCR. Results The coding sequence of ECSA gene was successfully subcloned in pRUF expression vector and transfected to packaging cells that the efficiency of transfection to GP293 was higher than the HEK293 cells. The enriched virus particles were obtained at a final concentration of 105 TU/ml. Conclusion Considering the critical characteristics of retroviral expression system such as stable and longtime expression of interested gene, being safe due to the deletion of retroviral pathogenic genes, and since the function of ECSA gene is not clear, we used this system to induce expression of ECSA and prepared a valuable platform to analyze the biological function of the gene. Also the recombinant ECSA protein can be used in production of recombinant vaccines and serological tests. Keywords: DPPA2, ECSA, Embryo/cancer gene, KYSE cell line, Retroviral expression system. |
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| کلیدواژه‌های انگلیسی مقاله |
poster presentation |
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| نویسندگان مقاله |
m خالقی زاده | m khaleghizadeh
department of microbiology, damghan branch, islamic azad university, damghan, iran.
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
mm فرقانی فرد | mm forghanifard
department of biology, damghan branch, islamic azad university, damghan, iran.
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
m غلامین | m gholamin
human genetic division, immunology research center, avicenna research institute, mashhad university of medical sciences, mashhad, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
b معمار | b memar
department of pathology, imam reza hospital, mashhad university of medical sciences, mashhad, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
mr عباس زادگان | mr abbaszadegan
human genetic division, immunology research center, avicenna research institute, mashhad university of medical sciences, mashhad, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
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| نشانی اینترنتی |
http://ijp.mums.ac.ir/article_2715.html |
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| زبان مقاله منتشر شده |
en |
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