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Journal of Medical Bacteriology، جلد ۳، شماره ۱-۲، صفحات ۵۲-۵۹
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عنوان انگلیسی Identification o f Clinical Methicillin and Mupirocin-resistant Staphylococcus aureus by Multiplex-PCR
چکیده انگلیسی مقاله Background : Infections resulted from multi-resistant Staphylococcus aureus are increasing worldwide. In the present study, a Multiplex-PCR assay for the detection of antibiotic resistance genes among S. aureus clinical isolates and for the concomitant identification of these isolates was described. Methods : A total of 127 S. aureus isolates were collected from clinical specimens at three teaching hospitals in Tehran, Iran. Screening for methicillin and mupirocin resistance in staphylococcal isolates was performed by disk diffusion method, according to Clinical and Laboratory Standards Institute (CLSI) guidelines. The presence of femB, mecA, and iles-2 genes was investigated by multiplex-PCR. Results : The 62.2% and 7.8% of Staphylococcus isolates were positive for mecA and ileS-2 genes, respectively. The femB fragment was amplified in all S. aureus strains tested. There is a 100% concordance between susceptibility and PCR results for isolates which harbored ileS-2. However, 55.1% of staphylococci were found as MRSA in the phenotypic assay. Conclusions : The PCR assay described in this study was able detect three genes for identification of S. aureus and its methicillin and mupirocin resistant genotypes concomitantly in a single reaction. Hence, this method can be used on regular basis as a valuable diagnostic and surveillance tool in clinical laboratories.
کلیدواژه‌های انگلیسی مقاله Methicillin-resistant,S. aureus,Mupirocin-resistance,PCR
نویسندگان مقاله اباذر پورنجف | abazar pournajaf
department of microbiology, faculty of medicine, iran university of medical sciences, tehran, ir iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

عبدالله اردبیلی | abdollah ardebili
department of microbiology, faculty of medicine, golestan university of medical sciences, gorgan, ir iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی گلستان (Golestan university of medical sciences)

عزت الله قایمی | ezzat allah ghaemi
department of microbiology, faculty of medicine, golestan university of medical sciences, gorgan, ir iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی گلستان (Golestan university of medical sciences)

سجاد امیدی | sajad omidi
department of pathobiology, department of microbiology, faculty of public health, tehran university of medical sciences. tehran, ir iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

کتایون برهانی | katayoun borhani
department of pediatrics, children’s medical center, tehran university of medical sciences, tehran, ir iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمود خدابنده | mahmoud khodabandeh
department of pediatric infectious disease, faculty of medicine, tehran university of medical sciences, tehran, ir iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

معصومه داورپناه | masoumeh davarpanah
department of microbiology, faculty of medicine, isfahan university of medical sciences, isfahan, ir iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

نشانی اینترنتی http://jmb.tums.ac.ir/index.php/jmb/article/view/22
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نوع مقاله منتشر شده Original Articles
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