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Journal of Medical Bacteriology، جلد ۵، شماره ۱, ۲، صفحات ۳۶-۴۲
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عنوان انگلیسی Phenotypic and Genotypic Detection of Metallo-beta-lactamases among Imipenem-Resistant Gram Negative Isolates
چکیده انگلیسی مقاله Background : Imipenem-resistant gram negative bacteria, resulting from metallo-beta-lactamase (MBLs)-producing strains have been reported to be among the important causes of nosocomial infections and of serious therapeutic problem worldwide. Because of their broad range, potent carbapenemase activity and resistance to inhibitors, these enzymes can confer resistance to almost all beta-lactams. The prevalence of metallo-beta-lactamase among imipenem-resistant Acinetobacter spp., Pseudomonas spp. and Enerobacteriaceae isolates is determined. Methods : In this descriptive study 864 clinical isolates of Acinetobacter spp., Pseudomonas spp. and Enterobacteriaceae , were initially tested for imipenem susceptibility. The metallo-beta-lactamase production was detected using combined disk diffusion, double disk synergy test, and Hodge test. Then all imipenem resistant isolates were tested by PCR for imp , vim and ndm genes. Results : Among 864 isolates, 62 (7.17 %) were imipenem-resistant. Positive phonetypic test for metallo-beta-lactamase was 40 (64.5%), of which 24 (17.1%) and 16 (9.2%) isolates were Acinetobacter spp. and Pseudomonas spp., respectively. By PCR method 30 (48.4%) of imipenem resistant Acinetobacter, and Pseudomonas isolates were positive for MBL-producing genes. None of the Enterobacteriaceae isolates were positive for metallo-beta-lactamase activity. Conclusion : The results of this study are indicative of the growing number of nosocomial infections associated with multidrug-resistant gram negative bacteria in this region leading to difficulties in antibiotic therapy. Thereby, using of phenotypic methods can be helpful for management of this problem.
کلیدواژه‌های انگلیسی مقاله Gram negative bacteria, Metallo beta lactamase, Imipenem
نویسندگان مقاله محمد محمد زاده | mohamad mohamad zadeh
1department of microbiology, school of medicine, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

مهناز توکلی | mahnaz tavakoli
1department of microbiology, school of medicine, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

ابوالفضل محبی | abolfazl mohebi
2department of microbiology, faculty of science, islamic azad university, karaj, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

صمد آقایی | samad aghayi
2department of microbiology, faculty of science, islamic azad university, karaj, iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

نشانی اینترنتی http://jmb.tums.ac.ir/index.php/jmb/article/view/183
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